| Abstract [eng] |
High voltage pulse generator for applications in cell electroporation research is analyzed in this master thesis paper. Electroporation research is important in a number of applications in medical treatment. The aim of the work is to suggest the optimal scheme of electroporator, to choose corresponding elements and to examine the parameters of constructed electroporator prototype. Effect of electroporation and types of electroporation pulse generators are reviewed in the first part of the work. Commercially available electroporators are also reviewed. Calculations of the main parameters of pulse generator are made in the second part of this work. Digital models of real Insulated gate bipolar transistor (IGBT) and pulse generator were made. Influence of various circuit parameters on the pulse shape was analyzed using these digital models. Method of square pulse turn-off time improvement was suggested. Measurements of the prototype generator parameters were conducted in the last part of the work. Measured pulses were analyzed and compared with modeled ones. Structure: introduction, literature review, designing of pulse generator, analysis of constructed generator, conclusions and suggestions, references. Thesis consists of 50 p. of text without appendixes, 31 figure, 10 tables, 25 bibliographical entries. |
